Losytskyy Mykhaylo Yuriyovych
PhD, researcher
Curriculum vitae
Studied in Kyiv National Taras Shevchenko University at physics department (1994-99) and as PhD student at experimental physics division (1999-2002).From 2002 till 2003 worked as leading engineer at Institute of Molecular Biology and Genetics of NAS of Ukraine; from 2003- as engineer in Kyiv National Taras Shevchenko University.
In 2005 has defended PhD theses “Spectral manifestation of interaction of cyanine dyes in water solutions and at the DNA surface".
From 2006 – junior researcher, from 2007 – researcher of experimental physics division at physics department of Kyiv National Taras Shevchenko University. Works also as assistant lecturer at the division of experimental physics and scientific collaboration in Institute of Molecular Biology and genetics of NASU.
Main directions of scientific investigations: investigation of fluorescent properties of dye molecules, photo-physical processes in this molecules, interaction of dyes with biological molecules (nuclear acids, proteins), electron excitation energy transfer in system DNA-dye, and the processes of dye molecules aggregation in solution and at the surface of biological molecules.
Main achieved results: determination of H-aggregation nature of long-wave fluorescence of some cyanine dyes in water solutions and in the presence of DNA; determination of the structure of electron energy levels and electron transitions of J-aggregate with Davydov splitting of absorption, selectively formed at AT-sequences of DNA; determination of the mechanism of linkage of the series of dye molecules with DNA.
Has 43 papers (total number of publications – over 100).
Publications
- M.Yu.Losytskyy, V.M.Yashchuk, S.M.Yarmoluk. Electronic singlet excitations transfer in DNA-cyanine dye system // Functional Materials. – 2000. – Vol.7, №4(1).
- S.M. Yarmoluk, M.Yu. Losytskyy, V.M. Yashchuk. Nonradiative deactivation of the el2. ectronic excitation energy in cyanine dyes: influence of binding to DNA // Journal of Photochemistry and Photobiology B: Biology. – 2002. – Vol. 67, №1.
- M.Yu. Losytskyy, V.M.Yashchuk, S.S.Lukashov, S.M.Yarmoluk. Davydov. Splitting in Spectra of Cyanine Dye J-Aggregates, Formed on the Polynucleotide // Journal of Fluorescence. – 2002. – Vol.12, №1.
- M.Yu.Losytskyy, K.D.Volkova, V.B.Kovalska, I.E.Makovenko, Yu.L.Slominskii, O.I.Tolmachev, S.M.Yarmoluk. Fluorescent Properties of Pentamethine Cyanine Dyes with Cyclopentene and Cyclohexene Group in Presence of Biological Molecules // Journal of Fluorescence. – 2005. – Vol.15, №6.
- N.Akbay, M.Yu.Losytskyy, V.B.Kovalska, A.O.Balanda and S.M.Yarmoluk. The Mechanism of Benzothiazole Styrylcyanine Dyes Binding with dsDNA: Studies by Spectral-Luminescent Methods // Journal of Fluorescence. – 2008. – V.18, Iss.1.
- K.D. Volkova, V.B. Kovalska, M.Yu. Losytskyy, L.V. Reis, P.F. Santos, P. Almeida, D.E. Lynch, S.M. Yarmoluk "Aza-substituted squaraines for the fluorescent detection of albumins" Dyes and Pigments, 2011. -V. 90,N1. - С. 41 - 47
- V.B. Kovalska, M.Yu. Losytskyy, S.M. Yarmoluk, I. Lubitz, A.B. Kotlyar "Mono and Trimethine Cyanines Cyan 40 and Cyan 2 as Probes for Highly Selective Fluorescent Detection of Non-canonical DNA Structures" Journal of Fluorescence, 2011. -V. 21,N1. - С. 223 - 230
- K.D. Volkova, V.B. Kovalska, M.Yu. Losytskyy, K.O. Fal, N.O. Derevyanko, Y.L. Slominskii, O.I. Tolmachov and S.M. Yarmoluk"Hydroxy and Methoxy Substituted Thiacarbocyanines for Fluorescent Detection of Amyloid Formations" Journal of Fluorescence, 2011. -V. 21,N2. - С. 775 – 784
- I. Madan, M. Losytskyy, V. Yashchuk, N. Gamaleia, O. Golovii "Influence of Surface-Active Stabilizers on Porphyrin – Gold Nanoparticles Absorption and Fluorescence" Molecular Crystals and Liquid Crystals, 2011. -V. 536,N1. - С. 17 - 23
- А.Gerasov, M.Shandura, Yu.Kovtun, M.Losytskyy, V.Negrutska, I.Dubey. Fluorescent labeling of proteins with amine-specific 1,3,2-(2H)-dioxaborine polymethine dye. Analytical Biochemistry, 2012. -V. 420,N2. - С. 115 – 120.
- O.A. Yeshchenko, I. S. Bondarchuk, M. Yu. Losytskyy, A. A. Alexeenko, “Temperature dependence of photoluminescence from silver nanoparticles”, Plasmonics, 2013, Published online: 20 July 2013, DOI: 10.1007/s11468-013-9601-3. I (F= 2.425).
- M.Y. Losytskyy, V.B. Kovalska, O.A. Varzatskii, A.M. Sergeev, S.M. Yarmoluk, Y.Z. Voloshin. Interaction of the Iron (II) Cage Complexes With Proteins: Protein Fluorescence Quenching Study // Journal of Fluorescence. – 2013. – Vol. 23, Iss. 5. – P. 889-895. (IF=1.88)
- O.A.Yeshchenko, I.S. Bondarchuk, M.Yu.Losytskyy. Surface plasmon enhanced photoluminescence from copper nanoparticles: Influence of temperature // Journal of Applied Physics. – 2014, Vol.116. – P.054309-1–054309-8.
- O.A.Yeshchenko, I.S.Bondarchuk, V.V.Kozachenko, M.Yu.Losytskyy. Surface Plasmon Enhanced Photoluminescence of Rhodamine 6G on Au Nanoparticles 2D Array: Temperature Effects // Proc. of 4th International Conference “Nanomaterials: Applications and Properties (NAP-2014)”, Lviv, Ukraine, September 21-27. – 2014. – Vol.3. – No.2. – P.02MAN04-1–02MAN04-4.
- M.Yu.Losytskyy, I.V.Madan, N.V.Kutsevol, M.M.Petrenko, V.M.Yashchuk. Effect of polyacrylamide and dextran-polyacrylamide graft polymers on absorption and fluorescence spectra of hematoporphyrin // Molecular Crystals and Liquid Crystals. – 2014. – Vol.589, Iss.1. – P.226-231.
- O.A.Yeshchenko, I.S.Bondarchuk, M.Y.Losytskyy, A.A.Alexeenko. Temperature Dependence of Photoluminescence from Silver Nanoparticles // Plasmonics. – 2014. – Vol.9, Iss.1. – P.93-101.
- O.A.Yeshchenko, I.S.Bondarchuk, V.V.Kozachenko, M.Yu.Losytskyy, Photoluminescence of rhodamine 6G in plasmonic field of Au nanoparticles: Temperature effects// Journal of Luminescence. – 2015. – V.158. – P. 294-300.
- O.A.Yeshchenko, I.S.Bondarchuk, V.V.Kozachenko, M.Yu.Losytskyy. Sensing the temperature influence on plasmonic field of metal nanoparticles by photoluminescence of fullerene C60 in layered C60/Au system // Journal of Applied Physics. – 2015. – V.117, P.153102-1 – 153102-2.